Western Blot analysis protocol(한글)

analysisof current actual network conditions. NOTE: this does notinclude cases of routing decisions taken on predefinedinformation. E EARN European Academic Research NetworkEBCDIC Extended Binary-coded Decimal Interchange CodeEGP Exterior Gateway ProtocolA protocol which distributes routing information to thegateways (routers) which connect autonomous systems.EthernetA network standard for the hardware and data link levels.There are two types of Ethernet: Digital/Intel/Xerox (DIX)and IEEE 802.3. F FDDI Fiber Distributed Data InterfaceFDDI is a high-speed (100Mb) token ring

Western and Eastern Europe and Mexico to share experiences. It emerged from the discussions that the way in which globalisation is taking place has had an extremely adverse effect on workers in general and women workers in particular. There has been a rapid informalisation of employment, which has resulted in falling real wages, erosion of labour rights, increased hazards and environmental degradation, longer hours of work, increasing quotas and growing poverty and unemployment. The human and social costs include the destruction of families and an erosion of the social fabric, dramatically ex

analysis of a nursing center’s services. NursingEconomics, 16(6), 307-312.Ervin, N. E., Nelson, L. L., & Sheaff, L. (1999). Pre-venting adverse outcomes: A populationfocus. Journal of Nursing Care Quality, 13(6),25-31.Ervin, N. E., & Young, W. B. (1996). Model for a nurs-ing center: Spanning boundaries. Journal ofNursing Care Quality, 11(2), 16-24.Freeman, R. B. (1950). Public health nursing. Phila-delphia: Saunders.Freeman, R. B. (1970). Community health nursingpractice. Philadelphia: Saunders.Gardner, M. S. (1926). Public health nursing (2nded.). New York: Macmillan.Glick,

protocol: 보관방법, origin어딘지, protein 특징 알아두기!)B-actin MYPT1Thr855-PCPI17Thr38-P => Total1°AB B-actin 1:2000, 시약 5ul+skin milk 10ml(2°AB anti-mouse IgG : HRP Conj. → stressgen)MYPT1 P 1:5000 2ul + 10ml (total 1:2000 5ul +10ml) (2°AB anti rabbit IgG : HRP-linked AB → Cell signaling)CPI17 1:250 20ul+5ml (total 1:1000 200ug/80ml(?) 5ul +50ml)-> 양이 많으므로 sealing, total 10ml에 해야 하니깐 5ml sealing 함 (?) (2°AB anti-goat → santa cruz, total → T up-stake) *B-acin→ 실내냉장고(왼), MYPT1→ deepfrazer(오.밑2), CPI17→실

1. 실험제목Western blot analysis using His-tag Antibody4. 목적Western Blot Analysis를 His-Tag antibody를 이용해 실험하고, 그 원리와 사용법을 안다.더 나아가, Antibody를 이용한 다른 여러 가지 analysis를 알아본다. 5. 원리1> western blotting 의 원리① 장점특정 단백질의 antigenicity(항원성)을 확인할 수 있음특정 protein 확인 가능size 별로 분리하므로 분자량 측정 가능② 기본적인 blotting 과정Preparation of the antigen sample Resolution of the sample by gel electrophoresis Transfer of the separ