[논문발표용PPT] Flavone inhibits vascular contraction by decreasing phosphorylation of myosin phophatase target subunit

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  • 2010.05.30 / 2019.12.24
  • 11페이지 / fileicon ppt (파워포인트 2003)
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하고 싶은 말
대학원 석사과정 때 위 논문을 읽고 발표한 PPT입니다.
PPT에 발표 스크립트도 포함되어 있습니다.
논문읽고 발표수업 하실 때 참고하시면 많은 도움 되실겁니다.
본문내용
◆ Material
- U46619, KCl
- isoflavone – genistein, daidzein

◆ Western blot Analysis
- Glycerol Urea PAGE : MLC phosphorylation
- SDS PAGE : CPI17Thr38, MYPT1Thr855

◆ GTP-RhoA assay

◆ Tension measurements
- organ bath (20ml)
- Animal : SD rat (10 week), Thoracic aorta (4mm in length)

참고문헌
Isoflavone attenuates vascular contraction through inhibition of RhoA/Rho-kinase signaling pathway-The Journal of Pharmacology and Experimental Therapeutics. 2008 by The American Society for Pharmacology and Experimental Therapeutics (JPET 326:991-998,2008)
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