Algae를 이용한 Biofuel 응용방안
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추천 연관자료
- 목차
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제 1장 문제에 대한 이해 및 목표 설정
제 2장 유전공학을 이용한 지질함량 증가
제 3장 바이오리액터를 이용한 대량생산 및 바이오디젤화 과정
제 4장 단계 실현화와 전망
- 본문내용
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Biodiesel을 얻기 위해 microalgae 사용
Microalgae에서 Oil의 함량을 높여야 함
TAG의 함량을 높여야 함
1) TAG biosynthesis pathway 관련 효소를 유전자수준에서 control
2) Bioreactor로 algae의 생장속도와 이차대사산물생성속도를 조절
<Nile Red를 이용한 lipid 측정방법>
Ultrapure Nile Red powder was dissolved in DMSO
(1 mg/mL) and stored at -20°C.
2) Working 2X (1 μg/mL) solutions were prepared in 50% DMSO.
3) Fluorescence was measured after 10 minute incubation at ambient temperature.
4) Fluorescence measurements were made from the top using 530nm excitation and 570 nm emission wavelengths.
특성이 기존 fossil diesel과 유사하여 서로 섞어
쓸 수 있다.
추가적인 공정을 거치면
① S 함량이 극도로 적고
② 에너지 효율이 여타 연료들
(Petroleum Diesel 등)보다 좋으며
③ greenhouse gas 방출량이 적어 훨씬
지속 가능 한 연료가 될 수 있다.
- 참고문헌
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World Nuclear Association. Another drop in nuclear generation World Nuclear News, 05 May 2010.
U.S. Energy Legislation May Be `Renaissance' for Nuclear Power.
Bernard Cohen. "The Nuclear Energy Option". Retrieved 2009-12-09.
"Nuclear Energy is not a New Clear Resource.". Theworldreporter.com. 2010-09-02.
Greenpeace International and European Renewable Energy Council (January 2007). Energy
Revolution: A Sustainable World Energy Outlook, p. 7.
Giugni, Marco (2004). Social protest and policy change: ecology, antinuclear, and peace movements in comparative perspective. Rowman & Littlefield. pp. 44?. ISBN 9780742518278.
Impact of biofuel production and other supply and demand factors on food price increases in 2008 , Sherry A. Mueller, James E. Anderson, Timothy J. Wallington,
Errol Kiong (May 12, 2006). "NZ firm makes bio-diesel from sewage in world first". The New Zealand Herald. Retrieved 2007-01-10.
Times, "Colorado Company to Take Algae-Based Fuel to the Next Level," 11 Nov 2008, M.L. Wald
Klass, Donald, "Biomass for Renewable Energy, Fuels, and Chemicals", page 341. Academic Press, 1998.
1062.
Kitani, Osamu, "Volume V: Energy and Biomass Engineering,CIGR Handbook of Agricultural Engineering", Amer Society of Agricultural, 1999.
"Biofuels: some numbers". Grist.org. Retrieved 2010-03-15.
Madrigal, Alexis (December 29, 2009). "How Algal Biofuels Lost a Decade in the Race to Replace Oil". Wired. Retrieved December 2009.
Madrigal, Alexis (December 29, 2009). "How Algal Biofuels Lost a Decade in the Race to Replace Oil". Wired. Retrieved December 2009.
Harvesting method : Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production(2008)
ASTM biodiesel evaluation : Production of Biodiesel from Algal Biomass: Current Perspectives and Future(School of Life Sciences, Tsinghua University, Beijing, People's Republic of China)
Kennedy pathway : Placing microalgae on the biofuels priority lists : a review of the technoogical challenges(2010)
Application : Microalgae for biodiesel production and other applications: A review(2010)
High-efficiency transformation of Chlamydomonas reinhardtii by electroporation
Cheney DP MB, and Stiller J: Agrobacterium-mediated genetic transformation in the macroscopic red alga Porphyra yezoensis. Journal of phycology 2001, 37(11)
Molnar A, Bassett A, Thuenemann E, Schwach F, Karkare S, Ossowski S, Weigel D, Baulcombe D: Highly specific gene silencing by artificial microRNAs in the unicellualr alga Chlamydomonas reinhardtii. Plant J 2009, 58(1): 165-74
High-efficiency transformation of Chlamydomonas reinhardtii by electroporation
Cheney DP MB, and Stiller J: Agrobacterium-mediated genetic transformation in the macroscopic red alga Porphyra yezoensis. Journal of phycology 2001, 37(11)
Baulcombe D. 2005. RNA silencing. Trends Biochem. Sci. 30: 290-293
Tang G. 2005. siRNAa nd miRNA: An insight into RISCs. Trends Biochem. Sci. 30: 106-114
Valencia-Sanchez M.A., Liu J., Hannon G.j, and Parker R. 2006. Control of translation and mRNA degradation by miRNAs and siRNAs. Gene Dev. 20: 515-524
E. D. Mullins, X. Chen, P. Romaine, R. Raina, D.M. Geiser, and S. Kang. 2001. Agrobacterium-mediated transformation of Fusarium oxysporum: An efficient too for insertional mutatgenesis and gene transfer. Phytopathology 91: 173-180
Bao X, Ohlrogge J: Supply of fatty acid is one limiting factor in the accumulation
of triacylglycerol in developing embryos. Plant Physiology 1999, 120(4):1057-
Biodiesel from microalgae
Biodiesel from oilgae, biofixation of carbon dioxide by microalgae: A solution
to pollution problems
Engineering algae for biohydrogen and biofuel production
Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga
Gene silencing by artificial microRNAs in Chlamydomonas
Genetic Engineering of Algae for Enhanced Biofuel Production
Lipid Biosynthesis
Manipulation of Microalgal Lipid
Production Using Genetic Engineering
Modifications of the metabolic pathways of lipid and triglyceride production in
microalgae
Second Generation Biofuels: High-Efficiency Microalgae
for Biodiesel Production
J. Vidal-Mas, O. Resina-Pelfort, E. Haba, J. Comas, A. Manresa & J.Vives-Rego(2001)
Rapid flow cytometry: Nile red assessment of PHA cellular content and heterogeneity in cultures of Pseudomonas aeruginosa 47T2 (NCIB 40044) grown in waste frying oil, Antonie van Leeuwenhoek 80: 57-63
Microfluidic Flow Cytometry: Principles of Cell Analysis and Applications
International Journal of Vascular Biomedical Engineering 2: 1-6 J. Vidal-Mas, O. Resina-Pelfort, E. Haba, J. Comas, A. Manresa & J.Vives-Rego(2001)
Molnar A, Bassett A, Thuenemann E, Schwach F, Karkare S, Ossowski S, Weigel D, Baulcombe D: Highly specific gene silencing by artificial microRNAs in the unicellualr alga Chlamydomonas reinhardtii. Plant J 2009, 58(1): 165-74
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